Approval of qPCR - A TaqMan real-time PCR (Than et al. 2013) for use in P. agathidicida surveillance and diagnostics
Document details the approval of the use of the TaqMan real-time PCR (qPCR) assay, originally developed by Than et al. (2013).
Purpose
This document describes the approval of the use of the TaqMan real-time PCR (qPCR) assay, originally developed by Than et al. (2013), for use in surveillance of Phytophthora agathidicida (PA) as a diagnostic test. This approval addresses a critical gap in diagnostic capability following the discontinuation of LAMP testing from the approved provider in 2024 and aims to strengthen New Zealand’s surveillance network through evidence-based molecular methods.
Approval to perform the qPCR testing for Tiakina Kauri is contingent on laboratories meeting containment requirements, following MPI-approved SOPs for soil sampling and baiting, and passing an MPI audit to ensure proficiency and cross-contamination control. Guidelines for reporting, interpretation, and handling unexpected positives are provided to ensure consistency and validity of results.