Standard Operating Procedures

Standard operating procedure for isolating Phytophthora agathidicida (PA) from soil.

Purpose This document describes the approval of the use of the TaqMan real-time PCR (qPCR) assay, originally developed by Than et al.

Standard operating procedure for sampling soil and roots to detect Phytophthora agathidicida (PA) .

The oDNA assay, published in 2025, introduces a novel DNA extraction approach that combines soil filtration with highly sensitive PCR, enabling direct testing of filtered soil.